Combination of Nigella sativa and Phyllanthus niruri as An Immunostimulant for The Prevention of White Spot Disease in Litopenaeus vannamei

Pacific whiteleg shrimp (Litopenaeus vannamei) is a very important commodity, which accounts for almost 70% of the total world production. However, this production still threatened by disease attacks, one caused white spot (WSD). Herbs are widely used as immunostimulants an effort to prevent diseases. Black cumin (Nigella sativa) and stone breaker (Phyllanthus niruri) have long been known excellent in human. This study aimed evaluate potential N. sativa its combination with P. niruri immunostimulant L. vannamei preventing WSD. Tested (±3.0 g) were treated (N), (P) both (1N:1P, 2/3N:1/3P, 3/4N:1/4P, 1/3N:2/3, 3/5N:2/5P, 1/4N: 3/4P, 2/5N:3/5P) through feed 28 days. They then infected syndrome virus (WSSV) observed 7 The parameters included immune response performance. was able reduce mortality test WSSV, RPS value 71%. can be combined right composition (1/2N:1/2P 2/3N:1/3P), while other combinations antagonistic. administration best treatments study, relatively did not affect hemocyte count, phenoloxidase activity, respiratory burst hemolymph clotting time performance tested shrimp.